This protein A purified antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 164 amino acids of the mature swine IFN beta protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Swine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000, WB: 1:500-1:2,000
Application Notes:
This protein A purified IFN-beta antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.5 kDa in size corresponding to swine IFN beta by western blott
Western blot using Rocklands protein-A purified anti-swine IFN beta antibody shows detection of recombinant Swine IFN beta at about 19.5 kDa raised in yeast. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) for 45 min at room temperature.
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