This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.
Conjugation:
Unconjugated
Alternative Names:
goat anti-HR23A Antibody, hHR23A antibody, MGC111083 antibody, RAD23 homolog A antibody, RAD23 yeast homolog A antibody, RAD23A antibody, UV excision repair protein RAD23 homolog A
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to HR23A by western blotting in the app
Western blot showing Rocklands Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Lane 1: HeLa nuclear extract (p/n W09-001-367). Lane 2: HeLa (p/n W09-000-364). Lane 3: A431 (p/n W09-000-361). Lane 4: Jurkat (p/n W09-001-370). Lane 5: 293 whole cell lysates (p/n W09-000-365). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG (p/n 605-4302) and chemiluminescence reagent with a 30-min exposure time. Other detection systems will yield similar results.
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