0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1 µg/mL
Application Notes:
Anti-gp120 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 98 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of gp120 antibody. Lane A: 25 ng of gp120 at 1 µg/mL. Lane B: 100 ng of gp120 at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 98 kDa, ~120 kDa for gp120.
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