ChAT (Choline Acetyltransferase) Antibody, Goat, Polyclonal

Product Overview

• Article Name: ChAT (Choline Acetyltransferase) Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-600-101-D16
• Supplier Catalog Number: 600-101-D16
• Alternative Catalog Number: ROC-600-101-D16
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Aves, Gallus, Guinea pig, Human, Mouse, Primate
• Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.
• Conjugation: Unconjugated
• Alternative Names: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Product Properties

• Clonality: Polyclonal
• NCBI: 1103
• UniProt: P28329
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:100, WB: 1:100
• Application Notes: Anti-ChAT antibody is tested by western blot and immunohistochemistry and suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by W

Images

Western Blot of Goat Anti-Choline Acetyltransferase Antibody. Lane 1: rat brain lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: ChAT antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) goat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~70kDa/~70kDa for ChAT. Other band(s): none.