Anti-PI3 Kinase p55 gamma Antibody, Goat, Polyclonal

Product Overview

• Article Name: Anti-PI3 Kinase p55 gamma Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-600-101-ML2
• Supplier Catalog Number: 600-101-ML2
• Alternative Catalog Number: ROC-600-101-ML2
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Anti-PI3 Kinase p55 gamma antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-Terminal portion of human PI3 Kinase p55 gamma conjugated to Keyhole Limpet Hemocyanin (KLH).
• Conjugation: Unconjugated
• Alternative Names: Goat Anti-PI3 Kinase p55 gamma Antibody, Goat Anti-PIK3R3 Antibody, Anti-p55 gamma Antibody, Phosphoinositide-3-Kinase Regulatory Subunit 3, Phosphatidylinositol 3-Kinase 55 KDa Regulatory Subunit Gamma, Phosphoinositide-3-Kinase, Regulatory Subunit 3 (Gamma), PtdIns-3-Kinase Regulatory Subunit P55-Gamma, PI3-Kinase Regulatory Subunit Gamma, PI3K Regulatory Subunit Gamma, PI3-Kinase Subunit P55-Gamma, P55PIK, Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 3 (P55, Gamma), Phosphatidylinositol 3-Kinase Regulatory Subunit Gamma, Phosphoinositide-3-Kinase Regulatory Subunit Gamma, PtdIns-3-Kinase Regulatory Subunit Gamma, P55-GAMMA, P55

Product Properties

• Clonality: Polyclonal
• Concentration: 1.02 mg/ml by UV absorbance at 280 nm
• NCBI: 8503
• UniProt: Q92569
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 50,000, IHC: 1:100, IF Microscopy: 15 µg/ml, WB: 1:1000
• Application Notes: Anti-PI3 Kinase p55 gamma Antibody has been tested in WB, IF, and IHC. Expect bands ~44, 47, 54kDa in western blot using appropriate tissues or lysates. Positive control used: mouse testis in WB, A431 cells in IF, Human testis and Human tonsil tissue in

Images

Immunohistochemistry of Goat anti-PIK3R3 Antibody. Tissue: Human Testes. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 minutes. Primary Antibody: Anti-PI 3 Kinase p55 gamma antibody at 1:100 for 30 minutes at RT. Secondary Antibody: Ready-to-Use Donkey anti-Goat HRP. Counterstain: Hematoxylin. Substrate: DAB. Results: PIK3R3 shows diffuse weak cytoplasmic expression in human testis for all germ cells and Leydig cells.

Immunofluorescence of Goat Anti-PIK3R3 Antibody. Cell Line: A431 Cells. Fixation: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-PI 3 Kinase p55 gamma Antibody at 15µg/mL overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG DyLight(TM)488 (p/n 605-741-125) at 15µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Localization: Cytosol (UniProtKB), Nucleoplasms (HPA) distinct punctate staining in nucleus could indicate nucleoplasm. Staining: (A) DAPI, (B) Anti-PIK3R3 + secondary, (C) A+B merge, (D) secondary only.

Western Blot of Goat Anti-PIK3R3 Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500) . Lane 2: PIK3R3 overexpressing lysate HEK293T lysate [+]. Lane 3: HEK293T Empty vector lysate [-]. Primary Antibody: Anti-PI 3 Kinase p55 gamma antibody at 1:1000 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n 605-703-002) at 1:40,000 for 30 minutes at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~54kDa overexpressed. ~44, 47, 54kDa endogenous human. Observed MW: ~57-65kDa. Exposure: 75 sec.

Immunohistochemistry of Goat anti-PIK3R3 Antibody. Tissue: Human Tonsil. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 minutes. Primary Antibody: Anti-PI 3 Kinase p55 gamma antibody at 1:100 for 30 minutes at RT. Secondary Antibody: Donkey anti-Goat HRP at 4µL/mL for 2 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Results: PIK3R3 showed universal tonsillar staining with increased cytoplasmic expression in germinal centers.

Western Blot of Goat Anti-PIK3R3 Antibody. Lane 1: Mouse Testes Lysate. Primary Antibody: Anti-PI 3 Kinase p55 gamma antibody at 1:1000 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n 605-703-125) at 1:5000 for 30 minutes at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~54kDa mouse. Observed MW: ~50kDa. Exposure: 1 sec.