Anti-NEUROD1 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region of human Neurogenic differentiation factor 1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugation:
Unconjugated
Alternative Names:
Goat Anti-Neuronal Differentiation 1 Antibody, Neurogenic Helix-Loop-Helix Protein NEUROD, Class A Basic Helix-Loop-Helix Protein 3, Neurogenic Differentiation Factor 1, Beta-Cell E-Box Transactivator 2, NEUROD, BHLHa3, Basic Helix-Loop-Helix Transcription Factor, Neurogenic Differentiation 1, NeuroD1, BHLHA3, NeuroD, BETA2, BHF-1, MODY6
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, WB: 5 µg/ml
Application Notes:
Anti-NEUROD1 Antibody has been tested in Western Blot. Expect a band at ~39.9 kDa in western blot using appropriate lysates or tissues. Positive control used rec. NEUROD1 protein or SCLC-21H whole cell lysates in WB.
Western Blot of Goat Anti-NEUROD1 Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HEK293 whole cell lysate spiked with NEUROD1 recombinant protein (35µg/50ng) [+]. Lane 3: HEK293 whole cell lysate (p/n W09-000-365) (35µg) [-]. Lane 4: SCLC-21H whole cell lysate (35µg) [+]. Primary Antibody: Anti-NEUROD1 at 5µg/mL overnight at 2-8. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n 605-703-125) at 1:40,000 for 30 min at RT. Blocking: BlockOut Buffer (p/n MB-073). Expected endogenous MW: ~39.9kDa. Expected recombinant MW: ~50-55kDa. Observed MW: ~50kDa.
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