Biotin Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal

Product Overview

• Article Name: Biotin Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal
• Biozol Catalog Number: ROC-600-103-098
• Supplier Catalog Number: 600-103-098
• Alternative Catalog Number: ROC-600-103-098
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: DOT, ELISA
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Conjugation: HRP
• Alternative Names: goat anti-Biotin Antibody HRP Conjugation, Peroxidase conjugated goat anti-Biotin Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:140,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000
• Application Notes: Anti-Biotin Peroxidase conjugated antibody has been tested by ELISA and dot blot. This product is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody

Images

ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 µg of Bovine Albumin Biotin Conjugated (p/n 001-0633). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.