GFP Antibody Peroxidase Conjugate, HRP, Goat, Polyclonal

Product Overview

• Article Name: GFP Antibody Peroxidase Conjugate, HRP, Goat, Polyclonal
• Biozol Catalog Number: ROC-600-103-215
• Supplier Catalog Number: 600-103-215
• Alternative Catalog Number: ROC-600-103-215
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Other
• Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Conjugation: HRP
• Alternative Names: goat anti-GFP Antibody peroxidase Conjugation, HRP conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 42212
• UniProt: P42212
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,000, WB: 1:2,000 - 1:5,000
• Application Notes: Polyclonal anti-GFP is designed to detect GFP and its variants. Anti-GFP Peroxidase conjugated antibody has been tested by ELISA to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enha

Images

Western Blot of Goat anti-GFP antibody Peroxidase conjugated. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: HEK293 lysate (p/n W09-000-365). Lane 3: HeLa Lysate (p/n W09-000-363). Lane 4: CHO/K1 Lysate (p/n W07-000-357). Lane 5: C2C12 Lysate. Lane 6: NIH/3T3 Lysate (p/n W10-000-358). Lane 7: Mouse Embryonic Fibroblast Lysate (p/n W10-001-371). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: 2 Epitope MBP Tag Marker Lysate (p/n MB-301-0100). Lane 10: Recombinant Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein (p/n 000-001-385). Marker: Opal Pre-stained ladder Load: 10 µg of lysate or 50ng of purified protein per lane. Primary antibody: goat anti-GFP peroxidase conjugated antibody at 1:1,000 overnight at 4C. Secondary antibody: none Blocking Buffer: BlockOut (p/n MB-073) for 30 min at RT. Predicted/Observed size: 28 kDa for GFP.