GFP (GOAT) Antibody Biotin Conjugated, Goat, Polyclonal

Catalog Number: ROC-600-106-215
Article Name: GFP (GOAT) Antibody Biotin Conjugated, Goat, Polyclonal
Biozol Catalog Number: ROC-600-106-215
Supplier Catalog Number: 600-106-215
Alternative Catalog Number: ROC-600-106-215
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Other
Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Conjugation: Biotin
Alternative Names: goat anti-GFP Antibody biotin Conjugation, biotin conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 42212
UniProt: P42212
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:50,000 - 1:80,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Application Notes: Anti-GFP Biotin Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-t
ELISA Results of Goat Anti-GFP Antibody tested against purified GFP protein. Each well was coated in duplicate with 10µg/mL of GFP protein (p/n 000-001-215). The starting dilution of antibody was 5µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and SA-HRP conjugated (p/n S000-03).