GFP Antibody Fluorescein Conjugated, Clone: [9F9.F9], FITC, Mouse, Monoclonal

Product Overview

• Article Name: GFP Antibody Fluorescein Conjugated, Clone: [9F9.F9], FITC, Mouse, Monoclonal
• Biozol Catalog Number: ROC-600-302-215
• Supplier Catalog Number: 600-302-215
• Alternative Catalog Number: ROC-600-302-215
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: DOT, WB
• Species Reactivity: Other
• Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Conjugation: FITC
• Alternative Names: mouse anti-GFP antibody FITC conjugation, fluorescein conjugated mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Product Properties

• Clonality: Monoclonal
• Clone Designation: [9F9.F9]
• NCBI: 42212
• UniProt: P42212
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:40,000, IF Microscopy: 1:500 - 1:2,500, WB: 1:10,000-1:25,000
• Application Notes: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited

Images

Western Blot of GFP Antibody Fluorescein Conjugated. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein Conjugated Mouse Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.