Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Conjugation:
Unconjugated
Alternative Names:
rabbit anti Biotin Antibody, rabbit anti-biotin Antibody
Clonality:
Polyclonal
Concentration:
1.3 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:200,000, IHC: User Optimized, WB: 1:2,000 - 1:10,000
Application Notes:
Anti-Biotin Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA Results of purified Rabbit Anti-Biotin Antibody tested against BSA-Biotin of immunogen. Each well was coated in duplicate with 1.0µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Goat anti-Rabbit IgG HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).
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