Alpha-1-Anti-Trypsin Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Alpha-1-Anti-Trypsin Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-147S
• Supplier Catalog Number: 600-401-147S
• Alternative Catalog Number: ROC-600-401-147S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Human Alpha-1-anti-Trypsin
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-alpha-1-antitrypsin antibody, A1A antibody, A1AT, AAT, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha-1-AT antibody, alpha1 PI antibody, alpha1 proteinase inhibitor antibody, Serpin A1, Anti-alpha-1-antitrypsin

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0mg/mL by UV absorbance at 280 nm
• NCBI: 5265
• UniProt: P01009
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:600,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody has been tested by ELISA, and western blot. This product is assayed against 1.0 µg of Human Alpha-1-anti-Trypsin in a standard ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as

Images

Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin (green), and Mouse-a-GST (blue) were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: Donkey anti-Rabbit IgG DyLight 549 (p/n 611-742-127), Donkey anti-Mouse IgG DyLight 488 (p/n 610-741-002), and Donkey anti-Goat IgG DyLight 649 (p/n 605-743-002) secondary antibody at 1:10,000 for 30 min at RT. Block: (p/n MB-070) at 30 min RT.