cdc2 phospho Y15 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: cdc2 phospho Y15 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-160
• Supplier Catalog Number: 600-401-160
• Alternative Catalog Number: ROC-600-401-160
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Xenopus
• Immunogen: Anti-cdc2 pY15 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues surrounding Tyr 15.
• Conjugation: Unconjugated
• Alternative Names: CDC-like kinase 2, Clk2, Cyclin-dependent kinase 1, Cdc2, Cdc2a, Cdkn1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase

Product Properties

• Clonality: Polyclonal
• NCBI: 54237
• UniProt: P39951
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, WB: 1:1000
• Application Notes: Anti-cdc2 pY15 antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 34 kDa in size corresponding to cdc2 protein in the appropriate cell lysate or

Images

Western Blot of Anti-cdc2 pT15 (Rabbit) Antibody. Lane 1: human T47D cells. Lane 2: human T47D cells with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2. Lane 3: human T47D cells incubated in lambda-Ptase (1200 units for 30 min). Lane 4: human T47D cells incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: cdc2 pT15 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~34kDa/~34kDa for cdc2 phosphorylated at Tyr15. Other band(s): none.