This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:425,000, ChIP: User Optimized, WB: 1:500 - 1:3,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the a
Western blot using Rocklands affinity purified anti-LDB1 antibody shows detection of LDB1 protein in Jurkat whole cell lysate (W09-001-370).Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO (p/n B501-0500) in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-145-122) for 45 min at room temperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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