This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-PPAR gamma 2 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma, PPARG2 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:4,000 - 1:40,000, WB: 1:1,000 - 1:3,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 55 kDa in size corresponding to PPARg2 by western blot in the
Western blot using Rocklands affinity purified anti-PPARg2 antibody shows detection of PPAR gamma 2 protein in a mouse 3T3 whole cell lysate (p/n W10-000-358). Approximately 20 µg of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2,000 dilution in 5% BLOTTO PBS solution (p/n B501-0500). The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.
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