RFA2 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: RFA2 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-448
• Supplier Catalog Number: 600-401-448
• Alternative Catalog Number: ROC-600-401-448
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Yeast
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 110-135 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively. Yeast RFA2 is phosphorylated at the G1/S transition and is dephosphorylated at mitosis.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-RFA2 antibody, RFA-2, RFA 2, Replication factor A protein 2, RF-A protein 2, Replication protein A 36 kDa subunit, DNA-binding protein BUF1, BUF-1, YNL312W, N0368

Product Properties

• Clonality: Polyclonal
• Concentration: 0.12 mg/mL by UV absorbance at 280 nm
• NCBI: 855404
• UniProt: P26754
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Yeast
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1000 - 1:3000, IP: 1:100, WB: 1:100 - 1:500
• Application Notes: This affinity-purified antibody was tested for use in western blotting against RFA2 containing cell lysates and by ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.

Images

This product was assayed by western blotting against RFA2 containing cell lysates. Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive Lot 13229 at 1.0 ug/ml followed by 1:5000 dilution of IRDye800 Sheep anti Rabbit IgG (lot 13218) for 1 hr at RT.