SH3BP2 phospho S427 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: SH3BP2 phospho S427 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-449
• Supplier Catalog Number: 600-401-449
• Alternative Catalog Number: ROC-600-401-449
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP3 protein (SH3 Domain Binding Protein 2).
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-SH3BP2 pS427 antibody, phosphorylated SH3BP2 antibody, SH3 domain-binding protein 2, SH3BP2 Src homology 3 Binding Protein 2, 3BP-2, RES4-23, 3BP2

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 10825
• UniProt: P78314
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SH3BP2 by western blotting in the ap

Images

Western blot analysis is shown using Rocklands Affinity Purified anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.