This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-BACH1 antibody, BACH-1, BACH 1, Fanconi anemia group J protein, FACJ, BRAC 1 Associated C Terminal Helicase 1 antibody, BRCA 1 Interacting Protein 1 antibody, BRCA1 binding helicase like protein BACH1 antibody, BRCA1 interacting protein C terminal helicase 1 antibody, BRIP 1 antibody, ATP-dependent RNA helicase BRIP1, FANCJ, FANC-J, FANC J
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:44,000, WB: 1:500- 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 105 -140 kDa in size corresponding to isoforms of BACH1 protein by west
Western blot using Rocklands Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kDa (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (p/n W09-000-365). Approximately 35 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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