This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-TLK1 antibody, TLK-1, TLK 1, PKU-beta antibody, serine threonine protein kinase antibody, Serine/threonine protein kinase tousled like 1 antibody, Tousled like kinase 1 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, WB: 1:200 - 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1
Western blot using Rocklands Affinity Purified anti-Tlk-1 antibody shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1. Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract (p/n W09-001-367) was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results
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