This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, IF Microscopy: User Optimized, WB: 1:500 - 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to UBP43 by western blotting in the app
Western blot using Rocklands Affinity Purified anti-UBP43 antibody. Lane 1: HeLa nuclear extracts (p/n W09-001-367). Lane 2: HeLa whole cell lysates (W09-000-364). Lane 3: A431 whole cell lysates (p/n W09-000-361). Lane 4: Jurkat whole cell lysates (W09-001-370). Lane 5: HEK293 whole cell lysates (p/n W09-000-365). Load: 18 µg. Primary Antibody: anti-UBP43 at 1:500. Shows detection of a band ~43 kDa band (arrowhead) believed to be to UBP43 in lysates. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.
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