This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human ßTrCP2 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-beta TrCP2 antibody, rabbit anti-BTRCP2 antibody, F-box/WD repeat-containing protein 11, beta Transducin Repeat Containing Protein 2 antibody, BTRC2 antibody, F-box and WD-40 Domain Protein 1B antibody, F-box Protein FBW1B antibody, FBW1B antibody, FBXW1B antibody, Homologous to Slimb protein, HOS, FBXW11
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:100,000, IF Microscopy: User Optimized, WB: 1:200 to 1:1,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting.
Western blot using Rocklands affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 (p/n W10-000-358) [lane 1] and 293 (p/n W09-000-365) [lane 2] whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n 611-103-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 1 h at 4 C.
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