This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal near aa 75-100 of human SmarcAL1 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-SMARCAL1 antibody, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA Related Protein antibody, hHARP antibody, SMARCA like Protein 1 antibody, Sucrose nonfermenting protein 2 like 1 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:16,000 - 1:64,000, WB: 1:500 - 1:3,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 106 kDa in size corresponding to SmarcAL1 by western blotting in the
Western blot using Rocklands Affinity Purified anti-SmarcAL1 antibody shows detection of a band ~106 kDa band corresponding to SmarcAL1 in human derived cultured cell lysates. Lane 1: HeLa nuclear extract (p/n W09-001-367), Lane 2: HeLa WC (p/n W09-000-364), Lane 3: A431 WC (p/n W09-000-361), Lane 4: Jurkat WC (p/n W09-001-370), and 293 WC (p/n W09-000-365). Approximately 5µg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.
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