This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 995-1009 of human FANCA protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-FANCA antibody, FANC-A, FANC A, Fanconi anemia group A protein, FAA antibody, FACA antibody, FAH antibody, FANCH antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:15,000 - 1:60,000, WB: 1:500 - 1:3,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a main band at approximately 163 kDa in size corresponding to FANCA by western blotting i
Western blot using Rocklands affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corresponding to FANCA in HeLa whole cell lysates (p/n W09-000-364). The identity of the lower molecular weight bands is unknown but may represent breakdown products.Approximately 35µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye(TM)800 fluorescence images were captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Western blot using Rocklands affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.
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