This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 350-375 of the Human Rad 52 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-RAD52 antibody, RAD-52, RAD 52, DNA repair protein RAD52 homolog antibody, RAD 52 antibody, RAD52 homolog antibody, recombination protein RAD52 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
Application Notes:
This affinity purified antibody has been tested by WB and ELISA. Anti-RAD52 is useful in western blotting using HeLa nuclear extracts. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibo
Western blot analysis is shown using Rocklands Affinity Purified anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kDa is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:5,000 for 45 min. IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by
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