This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-25 of Human RAD54 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-RAD54 antibody, RAD-54, RAD 54, RAD54A, RAD-54A, RAD 54A, RAD54L, RAD-54L, RAD 54L, DNA repair and recombination protein RAD54-like antibody, hHR 54 antibody, hHR54 antibody, hRAD 54 antibody, hRAD54 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized, WB: User Optimized
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by western blotting in
The hRad54 complex and plasmid DNA are indicated by the shaded oval and black line, respectively. (A) Movement of the hRad54 complex by tracking along the helical path of DNA is indicated by the arrows. When the complex is free to rotate around the DNA, no change in supercoiling will be induced in the plasmid DNA. (B) When the hRad54 complex tracks along the helix, while being prevented from rotating around the DNA, positive supercoils will arise ahead of the protein complex and negative supercoils behind it. These supercoils can freely distribute along the plasmid and therefore they will cancel each other out. (C) The interaction of two hRad54 complexes on a plasmid will divide the plasmid into two domains. Because the plasmid is singly nicked, one domain will contain a nick, whereas the other contains two covalently closed DNA strands. Depending on the position of the nick relative to the movement of the protein complex along the DNA, topoisomers containing either negative or positive supercoils will result after ligation of the nick.
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