This anti-Swi6 Antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Saccharomyces pombe
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:30,000, WB: 1:500 - 1:3,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Swi6 protein by western blotting in
The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.
Western blot analysis is shown using Rocklands Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S.pombe Swi6 protein. ~35µg of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 µm nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:5,000 for 45 min. IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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