This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bands corres
Western blot using Rocklands Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kDa corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~ 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MXHu (611-432-122) for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (not shown). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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