This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-ASAP1 Antibody, ASAP-1, ASAP 1, Development and differentiation enhancing factor 1 antibody, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody, ADP ribosylation factor directed GTPase activating protein 1 antibody, AMAP 1 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000 - 1:10,000, IF Microscopy: User Optimized, WB: 1:500 - 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to ASAP1 protein by wes
Immunofluorescent microscopy using Rocklands Affinity Purified anti-ASAP1 antibody shows detection of ASAP1 present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
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