ECT2 phospho T790 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: ECT2 phospho T790 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-935
• Supplier Catalog Number: 600-401-935
• Alternative Catalog Number: ROC-600-401-935
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-ECT2 pT790 Antibody, ECT 2 antibody, ECT-2 protein antibody, Epithelial cell transforming sequence 2 antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 1894
• UniProt: Q9H8V3
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:40,000, IP: User Optimized, WB: 1:1,000 - 1:4,000
• Application Notes: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by western blotting in the appropr

Images

Western blot using Rocklands affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD.