This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human MAGP-2 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:20,000, WB: 1:250 - 1:1,000
Application Notes:
This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20-30 kDa in size corresponding to MAGP-2 protein by western blotting i
Western blot using Rocklands affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4 C. Personal Communication, Michael Birrer, CCR-NCI, Bethesda, MD
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