This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 430-465 of human ABCB6 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-ABCB6 Antibody, ABCB 6, ABCB-6, ABC transporter umat antibody, ABC14 antibody, ATP binding cassette sub family B member 6, mitochondrial precursor antibody, mitochondrial precursor ABC transporter 3, Mt-ABC transporter 3
This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 94 kDa in size corresponding to ABCB6 protein by western blotting in th
Western blot using Rocklands affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) showed no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag(TM) antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1,400. Personal Communication, Jill Paterson, CCR-NCI, Bethesda, MD.
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