This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, WB: 1:500- 1:2,000
Application Notes:
This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 125 kDa in size corresponding to hCas5 protein is expecte
Western blot using Rocklands affinity purified anti-hCas5 antibody. Lane 1: AS cell lysate transfected with empty vector. Lane 2: AS cell lysate transfected with hCas5 (predicted MW 125kDa). Lane 3: BE2 cell lysate that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4: human muscle tissue lysate that shows high levels of hCas mRNA. Shows detection of a band ~125kDa in size corresponding to hCas5 in lane 2 (arrowhead). Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.
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