This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human Myosin 1G protein.
This affinity-purified antibody has been tested for use in ELISA, western blotting and possibly in immunoprecipitation as well. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 100 kDa in size c
Western blot using Rocklands affinity purified anti-Myosin 1G antibody. Lane 1: Jurkat cell lysates (p/n W09-001-370) [+]. Lane 2: peripheral blood T cell lysates [+]. Lane 3: human spleen lysates [+]. Lane 4: 300.19 cell lysates [+]. Lane 5: 293 cell lysates (p/n W09-000-365) [-]. Shows detection of a band ~100kDa in size corresponding to Myosin 1G (arrowhead) in Myosin 1G positive whole cell lysates. Personal Communication. Stephen Shaw, NCI, Bethesda, MD.
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