This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.
Conjugation:
Unconjugated
Alternative Names:
Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, IP: User Optimized, WB: 1:1,000
Application Notes:
Ready-To-Use Anti-Pdcd4 antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-SMAD3 (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 52 kDa in size corresponding to Pdcd4 protein by we
Western Blot Results of Ready to Use Rabbit Anti-Pdcd4 Antibody. Lane 1: Opal Pre-stained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: HEK293T WC lysate (p/n W09-001-GX5). Lane 3: HeLa WC Lysate (p/n W09-000-364). Load: 10µL. Primary Antibody: Ready to Use Rabbit Anti-Pdcd4 Antibody 1:1000 overnight at 4C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30 min RT. Block: BlockOut Buffer (p/n MB-073). Expect: ~52 kDa. Observed: ~35kDa.
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