This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-275 of human DAXX protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-DAXX Antibody, BING2 antibody, BING-2, DAP 6 antibody, DAP6 antibody, Death associated protein 6 antibody, Death domain associated protein 6 antibody, Fas death domain-associated protein, ETS1-associated protein 1, EAP1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:7,000 - 1:45,000, IF Microscopy: User Optimized, WB: 1:200 - 1:2000
Application Notes:
This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to DAXX protein by western blotting in the
Western blot using Rocklands affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25,000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.
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