This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19 kDa in size corresponding to GPx4 protein by western blotting in
Western blot using Rocklands affinity purified anti-GPx4 to detect GPx4 in testis extract (arrow). Tissue extract (40 µg) was electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication, Dolph Hatfield, CCR-NCI, Bethesda, MD.
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