This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:15,000 - 1:50,000, WB: 1:500 - 1:10,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Smad3 protein by western blotting i
Western blot using Rocklands affinity purified anti-Smad3 to detect over-expressed Smad3 in 231 cells (lane 2). Lane 1 shows mock infection of 231 cells with lentiviral vector alone. The membrane was probed with the primary antibody at a 1:5,000 dilution. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. Personal Communication
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