This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the carboxy terminal portion of mouse Cybr.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-Cybr Antibody, Cytohesin-interacting protein, Cytohesin-binding protein HE, Cbp HE, Pleckstrin homology Sec7 and coiled-coil domains-binding protein, Pscdbp, Cytip
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:20,000, WB: 1:1,000
Application Notes:
This antibody has been tested for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting in the app
Western blot using Rocklands affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct western blot of wt splenocytes. Lane 6 shows direct western blot of knock out mouse. Personal Communication, V. Coppola, NCI, Bethesda, MD.
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