This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-ATR antibody, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, ACTR antibody, AIB1 antibody, amplified in breast cancer 1 antibody, CAGH16 antibody, CBP interacting protein antibody, CTG26 antibody, FRP1, FRP-1, FRP 1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:15,000 - 1:70,000, WB: 1:1,000 - 1:5,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 301 kDa in size corresponding to ATR by western blotting in the app
Western blot using Rocklands Anti-ATR antibody. Lane 1: HeLa cell nuclear extract (p/n W09-001-367). Lane 2: HeLa cell nuclear extract pre-incubated with the immunizing peptide (50 µg peptide for 1 h at room temperature followed by centrifugation). Goat serum was used at 5% for blocking. Primary antibody: Anti-ATR at 1:1,400 dilution. The arrowhead corresponds to ~301kDa ATR when compared to MW markers (Lane M).
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