THRA Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: THRA Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-A38S
• Supplier Catalog Number: 600-401-A38S
• Alternative Catalog Number: ROC-600-401-A38S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-THRA antibody, rabbit anti-Thyroid hormone receptor alpha antibody, Nuclear receptor subfamily 1 group A member 1, c-erbA-alpha, c-erbA-1, V-erbA-related protein 7, EAR7, ERBA1, NR1A1, THRA1, THRA2

Product Properties

• Clonality: Polyclonal
• Concentration: 0.89 mg/mL by UV absorbance at 280 nm
• NCBI: 7067
• UniProt: P10827
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:300,000, IHC: 1:200, IF Microscopy: 1:200, IP: 1:100, WB: 1:500 - 1:2,000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.

Images

Western blot using Rocklands affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA. Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.