This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Stat5a protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-STAT5 antibody, STAT-5, STAT 5, STAT5A, Signal Transducer and Activator of Transcription 5A antibody, STAT-5A, Transcription factor STAT5A antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:25,000, WB: 1:500 - 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5 protein by wes
Western blot using Rocklands affinity purified anti-STAT5 antibody shows detection of Stat5 protein (arrowhead) in HeLa cell extract (p/n W09-000-364). Primary antibody was used at a 1:600 dilution reacted overnight at 4C. Secondary antibody was used at 1:20,000. Molecular weight estimation was made by comparison to prestained MW markers. Identity of the upper and lower bands is unknown. Personal communication, Luanne Lukes, NCI, Bethesda, MD.
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