Sars Nucleocapsid Protein Antibody, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-600-401-A50
Supplier Catalog Number:
600-401-A50
Alternative Catalog Number:
ROC-600-401-A50
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IF
Species Reactivity:
Virus
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-Sars Nucleocapsid Protein Antibody, rabbit anti-Sars-CoV Nucleocapsid (N) Protein Antibody, N antibody, N structural protein antibody, COVID19 antibody, Nucleocapsid protein antibody, Nucleoprotein antibody, SARS coronavirus N protein antibody, SARS CoV-2 antibody, SARSCoV2 antibody, Severe acute respiratory syndrome antibody, A50 Antibody, A50 SARS, COVID
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
SARS
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, Flow Cytometry: User Optimized, IHC: User Optimized, IF Microscopy: User Optimized, WB: 1:500 - 1:2,000
Application Notes:
Anti-SARS-CoV-1 purified antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsi
Surface Plasmon Resonance (SPR) Data of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody. SARS-CoV-2 Nucleocapsid protein was immobilized on a carboxymethyldextran sensor chip. Ten (10) dilutions of Anti-SARS-CoV Nucleocapsid (N) Antibody (colors) were flowed across the chip at 30 µL/min with 240 s contact time and then 720 s dissociation time. Fitted line (black) constants are: kon = 2.5 x 104 M-1 s-1 and koff = 1.6 x 10-4 s-1. The Equilibrium Dissociation constant (KD) of the antibody on several assays was 6.5 nM. (Average primary antibody KD = 1 µM).
Fitting of Kinetic Data to bivalent binding site model. Steady state signals are fitted to a bivalent binding site model. A 4-parameter non-linear regression curve was used for the integration of the data. The Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Antibody (p/n 600-401-A50) presents a KD=3.5nM (affinity constant) to SARS-CoV Nucleocapsid (N) Protein.
ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Antibody. Each well was coated in duplicate with 50ng of SARS CoV-1 N [Red Line], SARS CoV-2 N [[Green Line], MERS N [Blue Line]. The starting dilution of antibody was 0.55µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1 N IC50: 5ng/mL, SARS CoV-2 N IC50: 6ng/mL, MERS N IC50: 67ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).
Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection. Vero E6 cells were either infected with the SARS-CoV-2 Washington isolate (INF) at an MOI of 0.1 or uninfected (UNI) for 24 hours. The cells were then fixed in 4% PFA and stained overnight at 4C with primary antibodies directed against SARS-CoV Nucleocapsid (N) at 1:1000 dilution. Imaged using an anti-rabbit secondary conjugated to AlexaFluor 568 [red] and Nuclear Counterstain DAPI [blue]. Image Courtesy of Mohsan Saeed Lab/Da-Yuan Chen, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.
ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Variants. Each well was coated in duplicate with 0.5 µg/mL of SARS CoV-2 Canonical Nc [Red Line], SARS CoV-2 Omicron Nc [Green Line], SARS CoV-2 Omicron BA.2 Nc [Blue Line]. The starting dilution of antibody was 0.55 µg/mL and the X-axis represents the Log10 of a 3-fold dilution This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-2 Nc IC50: 5 ng/mL, SARS CoV-2 Omicron Nc IC50: 6 ng/mL, SARS CoV-2 BA.2 Nc IC50: 7 ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).
Binding mode and kinetic analysis of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Antibody (p/n 600-401-A50). Using Octet R4, SARS COV-1N was immobilized at 20 µg/mL on an AR2G biosensor and exposed to Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Antibody over a range of 0.8 nm - 6.4 µM. Each curve represents the acquired signal of its protein concentration. The interaction fits to a bivalent binding site kinetic model. The association constant (ka=2.6x10 4 M -1 s -1) and dissociation constant (kd=8.9x10 -5 s -1) values are depicted in the graph. The assay was performed as per the manufacturers protocol.
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