This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-SMAD2 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:100,000, IP: User Optimized, WB: 1:1,000 - 1:3,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting i
Western blot using Rocklands affinity purified anti-Smad2 to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle, the next lane contains lysates of MDA-MB231 cells treated with TGF beta. Low levels of staining in control lanes correspond to detection of endogenous Smad2. Pre-incubation of the antibody with immunizing peptide (data not shown) completely blocks specific band staining. The blot presented is askew relative to the molecular weight markers. The expected MW for Smad2 is 52 kDa. The membrane was probed with the primary antibody at a 1:2500 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.
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