TLR9 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: TLR9 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-A69
• Supplier Catalog Number: 600-401-A69
• Alternative Catalog Number: ROC-600-401-A69
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human toll-like receptor 9 (TLR9) protein.
• Conjugation: Unconjugated
• Alternative Names: CD 289 antibody, CD289 antibody, TLR 9 antibody, Toll like receptor 9 antibody, Toll like receptor 9 isoform A precursor antibody, Toll like receptor 9 isoform B antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 54106
• UniProt: Q9NR96
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:40,000, IHC: 2 ug/ml, IF Microscopy: 20 µg/mL, WB: 0.5-2 ug/ml
• Application Notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~94 kDa in size corresponding to TL

Images

Western Blot of Rabbit Anti-TLR-9 Antibody. Lane 1: Jurkat whole cell lysate using 0.5 µg/ml. Lane 2: Jurkat whole cell lysate using 1.0 µg/ml. Lane 3: Jurkat whole cell lysate using 2.0 µg/ml. Load: 10 µg per lane. Primary antibody: TLR 9 antibody overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 116 kDa, ~94 kDa for TLR9. Other band(s): none.