Damage Related Autophagy Modulator Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Damage Related Autophagy Modulator Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-A70
• Supplier Catalog Number: 600-401-A70
• Alternative Catalog Number: ROC-600-401-A70
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human DRAM protein.
• Conjugation: Unconjugated
• Alternative Names: DRAM antibody, FLJ11259 antibody, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator, DRAM1

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 55332
• UniProt: Q8N682
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:40,000, Flow Cytometry: 20 µg/mL, IF Microscopy: 2-2.5 µg/mL, WB: 0.5 - 2 µg/ml
• Application Notes: This affinity purified antibody has been tested for use in ELISA, immunofluorescence, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~33 kDa in size corresponding to DR

Images

Western blot using Rocklands affinity purified anti-DRAM antibody shows detection of a predominant band at ~33 kDa corresponding to DRAM (arrowhead) in K562 whole cell lysate. The predicted MW of DRAM is 26 kDa. DRAM was detected using 0.5 µg/ml (lane A), 1.0 µg/ml (lane B) and 2 µg/ml (lane C) concentrations of primary antibody.