This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-POGZ antibody, pogo transposable element with ZNF domain antibody, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635, SUHW5, ZNF280E, ZNF635
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:100,000 to 1:400,000, WB: 1:2,000 to 1:20,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 155 kDa in size corresponding to Pogz by western blotting in the ap
Western blot using Rocklands affinity purified anti-Pogz antibody shows detection of Pogz protein ~155kDa (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000. Personal Communication, K.O. Gudmundsson and J. Keller, NCI, Frederick, MD.
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