Anti-Caspase-8 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the C-terminus human caspase-8 isoform E.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1 µg/mL
Application Notes:
Anti-Caspase-8 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 55 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Caspase-8 antibody in HT-29 cell lysate. Lane A: Caspase-8 antibody in the presence of blocking peptide. Lane B: Caspase-8 antibody in the absence of blocking peptide. Load: 35 µg per lane. Primary antibody: Caspase-8 antibody at 1 µg/mL for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 32 kDa for Caspase-8. Other band(s): Caspase-8 splice variants and isoforms.
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