Anti-Caspase-9 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the N-terminus of human Caspase-9.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, IP: User Optimized, WB: 1:1,000
Application Notes:
Anti-Caspase-9 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 46 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Caspase-9 antibody. Lane 1: HeLa whole cel lysate. Load: 35 µg per lane. Primary antibody: Caspase-9 antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 46.3 kDa, 46 kDa for Caspase-9.
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