Anti-CX3CL1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 18 amino acids near the N-terminus of human CX3CL1.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5000 - 1:20,000, IP: User Optimized, WB: 0.5-2 µg/mL
Application Notes:
Anti-CX3CL1 Antibody has been tested for use in ELISA, Western Blotting and Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 42 kDa in Western Blots of specific cell lysates and t
Western Blot of CX3CL1 antibody. Lane 1: C2C12 cell lysate with CX3CL1 at 0.5 mg/mL. Lane 2: C2C12 cell lysate with CX3CL1 at 1 mg/mL. Lane 3: C2C12 cell lysate with CX3CL1 at 2 mg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 42.2 kDa, 47 kDa for CX3CL1.
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