Anti-DRAM antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the C-terminus of human DRAM.
Conjugation:
Unconjugated
Alternative Names:
DRAM Antibody, DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator
Anti-DRAM Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 26 kDa in Western Blots of specific
Immunofluorescence Microscopy of DRAM antibody. Tissue: Human liver cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: DRAM antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: DRAM as red fluorescent signal.
Western Blot of DRAM antibody. Lane A: 293 cell lysate at 0.5 µg/mL. Lane B: 293 cell lysate at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 26.2 kDa, ~29 kDa for DRAM. Other band(s): DRAM splice variants and isoforms.
Immunohistochemistry of DRAM antibody. Tissue: Human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DRAM antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DRAM is cytoplasmic. Staining: DRAM as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of DRAM antibody. Tissue: Human Liver cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: DRAM antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: DRAM as red fluorescent signal.
Immunohistochemistry of DRAM antibody. Tissue: Human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DRAM antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DRAM is cytoplasmic. Staining: DRAM as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of DRAM antibody. Lane A: K562 cell lysate at 0.5 µg/mL. Lane B: K562 cell lysate at 1 µg/mL. Lane C: K562 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 26.2 kDa, ~29 kDa for DRAM.
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